The fungi isolate were identifed on the bases of colonial morphology followed by microscopic examination after lactophenol staining.
Lactophenol staining
Little portion of the fungal isolate was mixed in a drop of lactophenol cotton blue stain made on a clean grease free microscopic slide using sterile wireloop, a smear was made and covered with a sterile cover slip. It was observed under the microscope using x10 and x40 objective lenses for detection of features as hyphae, mycelium and spore (Cheesbrough, 2004).
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